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Image Search Results
Journal: Journal of Cellular and Molecular Medicine
Article Title: Single‐cell RNA‐seq reveals altered NK cell subsets and reduced levels of cytotoxic molecules in patients with ankylosing spondylitis
doi: 10.1111/jcmm.17159
Figure Lengend Snippet: Expression of cytolytic molecules in plasma of HCs ( n = 16) and AS patients ( n = 16). The plasma levels of granzyme A (A), granzyme B (B) and granulysin (C) were determined by enzyme‐linked immunosorbent assay. Pearson correlation analysis was performed between granzyme A (D), granzyme B (E), granulysin (F) and disease activity (BASDAI). Horizontal lines and error bars show the mean ± SEM. ** p < 0.01; *** p < 0.001; ns = not significant
Article Snippet: Plasma levels of cytotoxic granules, including GZMA, GZMB and
Techniques: Expressing, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Activity Assay
Journal: Journal of Nanobiotechnology
Article Title: Targeting CD39 boosts PD-1 blockade antitumor therapeutic efficacy via strengthening CD8 + TILs function and recruiting B cells in cervical cancer
doi: 10.1186/s12951-025-03500-0
Figure Lengend Snippet: CD39 inhibitor combined with PD-1 blockade improved therapeutic efficacy by promoting CXCL13 release and B cell activation . ( A ) Enzyme-linked immunosorbent assay (ELISA) was used to detect the content of CXCL13 in the serum samples of isotype, αPD-1, CD39i, and combined treatment groups of mice. ( B ) The content of CXCL13 in murine tumors was detected using ELISA. ( C ) Flow cytometry allowed detection of the proportion of CXCR5 + B cells in murine tumors. ( D - E ) Flow cytometry ( D ) and dot plots ( E ) showed the proportion of activated B cell infiltration in murine tumors. ( F ) The dot plot displayed the proportion of GCB cells in tumor B220 + B cells. ( G , H ) Dot plots showed the proportion of dark zone (DZ) ( G ) and light zone (LZ) ( H ) in GCB cells in mouse tumors. ( I , J ) Representative histograms ( I ) and summarized Mean Fluorescence Intensity (MFI) diagram ( J ) showed the expression of BCL6 in GCB cells. ( K ) The dot plot showed the proportion of plasma cells among CD19 + B cells of mouse tumors. ( L ) The dot plot displayed the proportion of memory B cells in mouse tumor B220 + B cells. The values were obtained using the Kruskal–Wallis test. * , P < 0.05; ** , P < 0.01; ***; P < 0.001; ns , not significant . Abbreviations: GCB, geminal center B cells; DZ, dark zone; LZ, light zone
Article Snippet: The sections were stained with a
Techniques: Drug discovery, Activation Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Fluorescence, Expressing, Clinical Proteomics